Fig. 3

Lung cancer-associated transcriptional changes. a PCA analysis of all transcripts detected in tumor-bearing and vehicle mouse muscle. b Hierarchical clustering separates tumor-bearing and vehicle mouse muscle based on differential transcriptome patterns. c Heat map of selected transcripts commonly associated with muscle atrophy. Trim63 was significantly upregulated in tumor-bearing mouse muscle p = 0.011. d Heat map of selected transcripts commonly associated with the hypoxia pathway. Of particular interest, 5 genes were significantly downregulated (Cdk20 p = 0.0004, Ccnd1 p = 0.0024, E2F6 p = 0.0392) or upregulated (Adm p = 0.0081, Lox p = 0.0095). e Heat map of select4ed transcripts commonly associated with the autophagy pathway. There were no significant differences in the expression of these genes. For all heat maps, features are sorted by the highest average of the tumor samples, from top to bottom. Values are the RPKM values, row normalized for each feature, and then log2 transformed. Red = high expression, green = low expression. Statistical significance was determined as p < 0.05 in multiple t test with Holm-Sidak multiple testing correction. n = 4 vehicle, 4 tumor