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Fig. 1 | Skeletal Muscle

Fig. 1

From: High-throughput proteomics fiber typing (ProFiT) for comprehensive characterization of single skeletal muscle fibers

Fig. 1

Distinct protein profiles between intact skeletal muscle tissue and separated fibers. a Venn diagram of protein groups identified in whole muscle lysates and pooled soleus muscle fibers (n = 48). b Jitter plot of skeletal muscle and fiber protein intensities (n = 48). Gray dots represent proteins identified in both skeletal muscle and fiber proteomes and blue labeled dots illustrate fiber-specific proteins. c Cumulative ranking of protein intensities for skeletal muscle (gray) and isolated fibers (blue; n = 48). d Number of proteins identified in 1 to 96 isolated muscle fibers from the soleus; W, whole skeletal muscle. eg Overview of the experimental ProFiT workflow. e Isolation of single muscle fibers by collagenase digestion and manual fiber separation. f Sample preparation via SP3 beads and mass spectrometric analysis using short LC-MS gradients. g Data analysis and pooling of fibers with the same myosin heavy chain (MyHC) protein content by an automated liquid handling station

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