Fig. 3

Isolated type IIb muscle fibers from EDL, TA, and Gast muscles exhibit distinct protein profiles. a Hierarchical clustering of significantly regulated proteins (ANOVA p value < 0.05; Table S4) between type IIb fibers (expressing the MYH4 isoform) from the EDL, TA, and Gast muscles. b Z-score distribution of proteins in clusters 1–4. Selected proteins are labeled next to the graphs. c Kinases in type IIb fibers with different expression profiles in the EDL, TA, and GAST; branched-chain ketoacid dehydrogenase kinase (BCKDK), phosphorylase b kinase regulatory subunit beta (PHKB), phosphorylase b kinase γ catalytic chain, skeletal muscle isoform (PHKG1), phosphorylase b kinase regulatory subunit α, skeletal muscle isoform (PHKA1), and α-protein kinase 3 (ALPK3). d Co-immunostaining of desmin (DES) and myosin-4 (MYH4) in muscle cryosections depicting the TA (above dashed line) and EDL muscle (below dashed line). Type IIb fibers are marked by “a” (TA) or “b” (EDL; scale bar = 50 μm). e Quantitative analysis of the desmin fluorescence signal in type IIb (MYH4) positive myofibers in the TA and EDL muscles. Significances were tested by two-sided t tests (n = 74)