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Fig. 2 | Skeletal Muscle

Fig. 2

From: Transgenic mice expressing tunable levels of DUX4 develop characteristic facioscapulohumeral muscular dystrophy-like pathophysiology ranging in severity

Fig. 2

All bi-transgenic ACTA1-MCM;FLExD mouse models show mosaic patterns of nuclear DUX4-FL expression. Tibialis anterior muscles were analyzed by IF for DUX4-FL protein expression over 28 days (moderate model, panels cj) or 9 days (severe model, panels ot). Muscles from the mild model, bi-transgenic ACTA1-MCM;FLExD without TMX (panels m and n), showed low steady levels of DUX4-FL immunostaining. Muscles from ACTA1-MCM mice treated with TMX at MD14 (panels a and b) or SD9 (k and l) showed no DUX4-FL signal and served as negative controls. Red, DUX4 IF; Blue, DAPI staining; Green, WGA staining. Scale bar = 50 μm

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