Fig. 8

Skeletal muscles from the mild and moderately affected FSHD-like mouse models show signs of dystrophic histopathology. Representative cryosections of tibialis anterior, gastrocnemius, soleus, and quadriceps of indicated transgenic animals were analyzed with H&E staining. Female (a–d) ACTA1-MCM mice treated with 1× 5 mg/kg TMX, (e–h) mild model bi-transgenic mice without TMX, and (i–x) moderate model bi-transgenic mice treated 1× with 5 mg/kg TMX. The TMX-injected ACTA1-MCM control mice were assayed at MD28. The TMX-injected bi-transgenic mice were assayed at (i–l) MD3, (m–p) MD6, (q–t) MD14, and (u–x) MD 28. All mice had performed the treadmill exhaustion analysis, as described, 2× per week starting the week prior to TMX injection. Scale bar = 100 μm