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Fig. 1 | Skeletal Muscle

Fig. 1

From: Exclusive vital labeling of myonuclei for studying myonuclear arrangement in mouse skeletal muscle tissue

Fig. 1

Confocal images of young adult RG STM muscles. a-d A maximum intensity projection of a whole-mount preparation. a BTX stain reveals AChR aggregates of three NMJs. b Myonuclei exclusively labeled with transgenic mCherry. c DAPI labeling of all nuclei in muscle tissue. d Composite image. Arrows indicate examples of myonuclei lying adjacent to, but not directly underneath, the AChR aggregates. e-h Single plane confocal image of a cross-section. e Myonuclei exclusively labeled with transgenic mCherry. Asterisks indicate examples of peripheral myonuclei. f Sarcolemmas stained with anti-dystrophin antibody reveal the boundaries of myofibers. g DAPI labeling of all nuclei in muscle tissue. Arrows indicate DAPI-labeled nuclei outside of the sarcolemma stain. h Composite image. Asterisks and arrows correspond to those in e and g. Scale bars 20 μm

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