Fig. 2From: Exclusive vital labeling of myonuclei for studying myonuclear arrangement in mouse skeletal muscle tissueMaximum intensity projection confocal images of isolated single myofibers of young adult RG EDL muscle. Outlines of the individual myofibers were imaged using background auto-fluorescence. a Image of the synaptic area of a myofiber. The subsynaptic myonuclei (arrow) are identified by their tight clustering near the surface of the myofiber. b Arrow points to an mCherry negative, DAPI-positive nucleus among three mCherry positive nuclei that all appear to be within the sarcolemma. Scale bar 20 μmBack to article page