Fig. 4

Vital images of the same STM NMJ from a young adult RG mouse 2 weeks apart. a, a’ Epifluorescent images of AChRs labeled with fluorescent BTX. b, b’ Epifluorescent images of myonuclei labeled with mCherry-tagged histone H2B protein. a, b Images of an NMJ during the first imaging session. a’, b’ Images of the same NMJ 2 weeks later. The arrows point to a single NMJ and its corresponding four subsynaptic nuclei. The subsynaptic nuclei are clearly visible in both b and b’, and do not appear to have undergone major positional changes. The slight differences in the appearance of the AChRs and myonuclei between the two imaging sessions can likely be accounted for by a slightly different stretching of the muscle in each session. Scale bar 20 μm