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Table 1 List of reagents and other resources used in this study

From: A reference single-cell transcriptomic atlas of human skeletal muscle tissue reveals bifurcated muscle stem cell populations

Reagents

Dispase II (neutral protease, grade II)

Sigma-Aldrich

04942078001

Collagenase D, from Clostridium histolyticum

Sigma-Aldrich

11088866001

Commercial kits

Chromium Single Cell 3' Library & Gel Bead Kit v2

10X Genomics

CG00052 (protocol)

Chromium Single Cell 3' Library & Gel Bead Kit v3

10X Genomics

CG000183 (protocol)

Deposited data

Human ligand-receptor database

[43]

https://www.ncbi.nlm.nih.gov/pubmed/26198319

Human scRNA-seq dataset

This paper

GSE143704

Mouse scRNA-seq dataset

[29]

GSE143437

Software packages and algorithms

Cell Ranger 3.1.0 (July 24, 2019)

10X Genomics

https://support.10xgenomics.com/single-cell-gene-expression/software/downloads/latest

Seurat 3.1.0

[14]

https://github.com/satijalab/seurat

Scanorama (online version as of 2019-11-19)

[13]

https://github.com/brianhie/scanorama

Harmony (online version as of 2019-11-19)

[54]

https://github.com/immunogenomics/harmony

biomaRt 2.43.1 (online version as of 2020-01-08)

[64]

https://bioconductor.org/packages/release/bioc/html/biomaRt.html

Gene Set Enrichment Analysis (4.0.3)

[65]

http://software.broadinstitute.org/gsea/index.jsp

Ingenuity Pathway Analysis (IPA, 2019-08-30)

QIAGEN

https://www.qiagenbioinformatics.com/products/ingenuity-pathway-analysis/