Fig. 1
From: Exercise promotes satellite cell contribution to myofibers in a load-dependent manner
Running pattern and muscle hypertrophy are load-dependent. a Experimental set-up. b Average running distances and c average external work per night throughout the exercise protocol (n = 16 mice per group). d Soleus weight normalized to tibia length. e Representative cross-sections stained for laminin (red) and f quantification (n = 7–8 mice per group) of fiber cross-sectional area distribution in soleus muscle. g Representative pictures of single myofibers isolated from soleus and (h quantification of fiber volume of 11–16 single myofibers per mouse (n = 4 mice per group). i Muscle force production by grip strength and j time-to-fatigue in ex vivo stimulated muscle. Statistics, one-way ANOVA test with Tukey correction for multiple comparisons (d, h, i, j) or two-way ANOVA test with a Bonferroni post hoc test (b, c, f, k). (*p < 0.05; **p < 0.01; ***p < 0.001). Each dot represents a single mouse (d, i, j) or muscle fiber (h). Bar graphs (d, i, j) and line graphs (b, c, f, k) represent mean ± SEM (error bars). Violin plot (h). Scale bars, 50 μm (e) and 25 μm (g). Pax7mTmG/+ female mice on a C57BL/6J background were used for all analyses