Fig. 4

Fluorescent nuclear protein propagation renders reporter mice inadequate to assess SC fusion in myofibers. a Nuclear H2B-nGFP expressing and nTom⁺ myoblast were seeded at 1:1 ratio and differentiated for 144 h. b Double positive nuclei for H2B-nGFP⁺ and nTom⁺ (marked in yellow) were assessed over time in relation to total nuclei (Hoechst⁺). c Representative pictures of H2B-nGFP⁺ and nTom⁺ myoblast co-cultures over time. d nTom⁺ and committed WT myoblast were seeded at a 1:4 ratio and differentiated for 144 h. e Double positive nuclei for nTom⁺ and Hoechst⁺ were assessed over time in relation to total nuclei (Hoechst⁺). f nTom intensity was measured 1 h after seeding (T0) and upon full differentiation (T144). Data is represented as percentage of nTom⁺ nuclei per intensity range in relation to the total nTom⁺ nuclei. g Representative pictures of WT and nTom⁺ myoblast co-cultures over time. Statistics, one-way ANOVA test with Tukey correction for multiple comparisons (*p < 0.05; **p < 0.01; ***p < 0.001). Bar graphs represent mean ± SEM (error bars). Scale bar, 50 μm