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Fig. 3 | Skeletal Muscle

Fig. 3

From: High-throughput screening identifies modulators of sarcospan that stabilize muscle cells and exhibit activity in the mouse model of Duchenne muscular dystrophy

Fig. 3

OT-9 increases sarcospan gene expression in mouse and human muscle cell lines and protein levels in human DMD myotubes. a C2C12 and b healthy human myotubes treated for 48 h with 5 μM of OT-9 and PC1-36 exhibit an increase in sarcospan gene expression. Gene expression was normalized to β-actin and vehicle-treated cells (0.1% DMSO). Human DMD myotubes treated with 5 μM of OT-9 or PC1-36 exhibit an increase in sarcospan protein levels as quantified by c an indirect sandwich ELISA and d immunoblot analysis. e Quantification of immunoblot results. Cells were treated on day 2 of differentiation and harvested after 48 h. R.U. = relative units normalized to protein concentration for the ELISA and GAPDH for the immunoblot analysis. Data represents individual replicates and mean value. n = 3–27. SSPN, sarcospan; R.U., relative units. *p < 0.05, **p < 0.01, ****p < 0.0001

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Skeletal Muscle

ISSN: 2044-5040

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