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Fig. 1 | Skeletal Muscle

Fig. 1

From: Dnmt3b regulates DUX4 expression in a tissue-dependent manner in transgenic D4Z4 mice

Fig. 1

Dnmt3b affects DUX4 expression in mESCs, but the Dnmt3bMommeD14 variant does not induce a more severe phenotype in FSHD mice. a Dnmt3b ChIP-qPCR analysis showed that Dnmt3b is enriched at the D4Z4 repeat array in mESCs. The error bars represent the standard error of the mean (SEM) for two technical replicates. b A successful knockdown of Dnmt3b was confirmed by western blot and RT-qPCR in the B4 and B6 cell lines. DUX4 transcript levels were significantly enhanced as determined by a Student’s t test. *P < 0.05; **P < 0.01; ****P < 0. 0001. Error bars represent the SEM from five technical replicates. c Body weight at postnatal day 15 was not changed between all genotypes. Each dot represents a single mouse (male mice in black; female mice in grey) and the N is depicted per genotype. Statistical analysis was performed by one-way ANOVA. WT = wild-type; 2.5 = D4Z4-2.5; MD14 = Dnmt3bMommeD14. d Sex distribution was significantly disturbed; fewer female mice were born from the cross-breedings. Statistical analysis was performed using a Pearson’s chi-squared test. **P < 0.01. e Genotype distribution (male mice in black; female mice in grey) was not disturbed as tested by a Pearson’s chi-squared test. The N for male and female mice is depicted for each genotype. WT = wild-type; 2.5 = D4Z4-2.5; MD14 = Dnmt3bMommeD14

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