Fig. 2
From: Dnmt3b regulates DUX4 expression in a tissue-dependent manner in transgenic D4Z4 mice
The Dnmt3bMommeD14 variant affects Dnmt3b protein levels but not Dnmt3b mRNA levels. Dnmt3b transcript levels were not affected by the Dnmt3bMommeD14 variant as measured by RT-qPCR in different skeletal muscles and non-muscle tissues (postnatal day 15). Each dot represents one mouse, and the error bars denote the SEM from the biological replicates. Statistical analysis was performed using a Student’s t test (testis, spleen) or Mann-Whitney U test (quadriceps and gastrocnemius muscle). n.s. = not significant. 2.5 = D4Z4-2.5; MD14 = Dnmt3bMommeD14. b Dnmt3b protein levels were slightly reduced in the testis of D4Z4-2.5/Dnmt3bMommeD14 mice at postnatal day 15. Emerin was used as a loading control. c Quantification of Dnmt3b protein levels of the upper and lower band showed a significant reduction in the Dnmt3b/Emerin ratio in the testis of D4Z4-2.5/Dnmt3bMommeD14 mice as determined by a Student’s t test. Each dot represents one mouse, and the error bars denote the SEM from the biological replicates. **P < 0.01. 2.5 = D4Z4-2.5; MD14 = Dnmt3bMommeD14