Fig. 1

Identification of small molecules that promote satellite cell proliferation. a Chemical screen experimental schematic outlining FACS isolation and compound library treatment of satellite cells. b Screening compound library subdivided into broad target classes. c Representative immunofluorescent images of FACS-sorted satellite cells from C57BL/6-β-actin-EGFP mice on 96-well plates cultured for 4 days and treated with vehicle, compound, or bFGF. Proliferation was assessed via high content imaging using GFP as a cell marker. Scale bars denote 100 μm. d Change in total satellite cell number after culture in the presence of DMSO (green), bFGF (red), or screening compounds (blue). Dashed lines indicated 1 standard deviation (red) or 2 standard deviations (green) above the global mean calculated across all plates. Compounds that fall below 1 standard deviation from the vehicle mean are depicted in shaded blue