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Fig. 2 | Skeletal Muscle

Fig. 2

From: Pro-myogenic small molecules revealed by a chemical screen on primary muscle stem cells

Fig. 2

Validation and dose-response of chemical screen hits. a STITCH interaction networks generated from the primary screen hit compounds. b Experimental schematic detailing the compound treatment and culture duration for dose-response analysis. c–e Dose-response curves for hits present in the primary compound library. Error bars indicate SD from at least three independent experiments. Statistical significance was evaluated by a one-way ANOVA followed by post hoc Welch’s t test (*p < 0.05, **p < 0.01, ***p < 0.001). ANOVA p values for the following compounds: CEP-701 = 1.82E−11, Sunitinib = 7.38E−11, and Jak3 VI = 2.43E−12. f FACS-sorted satellite cells, expanded in the presence of either vehicle, CEP-701 (50 nM), or bFGF (5 ng/mL) then differentiated for 5 days, retain the capacity to fuse and form multinucleated myotubes. Myosin heavy chain (MyHC) is stained in red while the nuclei are counterstained with Hoechst (blue). Scale bar represents 100 μm

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