Fig. 4

The proliferative effect of CEP-701 is specific to the satellite cell. a Culture of primary myoblasts in proliferation conditions with or without bFGF in the presence of 50 nM CEP-701 or 2000 nM sunitinib does not alter expansion. N.S., not significant by 1-way ANOVA. b Culture of primary interstitial fibroblasts with or without bFGF in the presence of 50 nM CEP-701 does not alter expansion. Culture without bFGF in the presence of 2000 nM sunitinib does not alter expansion. Culture with bFGF and 2000 nM sunitinib acts synergistically to increase expansion. Both myoblasts and fibroblasts were treated as in Fig. 2a to match the conditions to primary satellite cells. Error bars indicate SEM from 4 independent experiments. **p < 0.001 by 1 way ANOVA followed by unpaired two-tailed t test assuming unequal variance with Bonferroni correction for multiple comparisons. c Primary myoblasts differentiated for 48 h in the presence of 50 nM CEP-701 or 2000 nM sunitinib demonstrate increased fusion and spindle-like morphology. Green, Phalloidin-488; red, myosin heavy chain; blue, Hoechst. Scale bar represents 200 μm. d Quantification of the number of nuclei per cell or fused myotube after myoblast differentiation in the presence or absence of CEP-701 or sunitinib. ***p < 0.001. N.S., not significant by 1-way ANOVA followed by unpaired two-tailed t test assuming unequal variance with Bonferroni correction for multiple comparisons. e The total number of myoblast nuclei in differentiation conditions is not altered by the presence of 50 nM CEP-701 or 2000 nM sunitinib. Error bars indicate SEM from 4 independent experiments. N.S., not significant by 1-way ANOVA