Fig. 3

Acute pharmacodynamics of GSK978A and NR in MDX hindlimbs following eccentric challenge. a Schematic of acute study design in MDX mice over 5 days (D1-D5) showing timing of eccentric contractions (ECC), assessments of contractility (CON), termination and tissue harvest (TERM), and compound administration. b Comparison of maximal tetanic force produced in the same limb at baseline and following eccentric challenge (ECC). Contractility (CON) was assessed over the 5-day protocol (D1-D5). c Masses of gastrocnemius muscles harvested from challenged (right, R) and contralateral (left, L) limbs at the study conclusion. d Total and (e) relative NAD content of gastrocnemius muscles harvested from challenged and contralateral limbs. f Total and (g) relative polyamine content of gastrocnemius muscles harvested from challenged and contralateral limbs. h Normalization of total polyamine to NAD contents in challenged limbs compared to the contralateral side. N = 8 MDX mice per group. Error bars represent SEM. Significance was determined by one-way ANOVA with Tukey’s post hoc test (**p < 0.01, ***p < 0.001, ****p < 0.0001; ns, not significant or p value indicated)