Fig. 5

Time course of hindlimb contractile function following consecutive eccentric challenges. a Longitudinal tetanic force produced by the hindlimbs beginning at week 10 of treatment and including repeat bouts of eccentric challenge (arrows) and recovery. b High-resolution traces of tetani immediately before and 24 h after the first eccentric challenge. c Three-week longitudinal hindlimb force production normalized to pre-challenge levels after the first and (d) second successive eccentric challenges. N = 9-12 mice per group. Mice are MDX unless otherwise noted. Error bars represent SEM. Significance was determined by repeated measure two-way ANOVA with Tukey’s post hoc test (*p < 0.05, ***p < 0.001, ****p < 0.0001; ns, not significant compared to MDX vehicle controls)