Table 1 Antibody Cocktails
From: MyoSight—semi-automated image analysis of skeletal muscle cross sections
Primary antibody | Secondary antibody | |
|---|---|---|
Laminin (membrane borders) | Abcam, ab11575 Rabbit IgG 2 μg/ml | ThermoFisher, A-11035 Goat Anti-Rabbit IgG Alexa Fluor 546 1 μg/ml |
MHC I | DSHB BA-F8 Mouse IgG2b 20ug/ml | ThermoFisher, A-21242 Goat Anti-Mouse IgG2b Alexa Fluor 647 10 μg/ml |
MHC IIa | DSHB SC-71 Mouse IgG1 20 μg/ml | ThermoFisher, A-21121 Goat Anti-Mouse IgG1 Alexa Fluor 488 10 μg/ml |
MHC IIb | DSHB BF-F3 Mouse IgM 20 μg/ml | ThermoFisher, A-21044 Goat Anti-Mouse IgM Alexa Fluor 594 10 μg/ml |
Myonuclei | Invitrogen D3571 DAPI 1:500 dilution |