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Table 2 Characteristics of Freely Available Image Analysis Programs

From: MyoSight—semi-automated image analysis of skeletal muscle cross sections

 

MyoSight

MuscleJ

Open-CSAM

MyoVision

SMASH

Compatibility

PC/Mac

PC/Mac

PC/Mac

PC Only

PC Only

Platform

FIJI

FIJI

FIJI

Open Source

MATLAB

Image type

Original format/TIFF

Original format/TIFF

TIFF

TIFF

TIFF

Auto/semi-automated

Semi-automated

Automated

Semi-automated

Automated

Semi-automated

Manual corrections

+

+

+

Re-analysis with new analysis parameters

+

+

CSA measurements

+

+

+

+

+

Identifies fiber-types

+

+

+

+

Identifies perinuclei

+

+

+

Identifies central nuclei

+

+

+

Identifies satellite cells

+

Identifies vessels

+

Accepts Z-stacks

+

Number of channels

5

4

1

5

3

Result files automatically generated

+

+

+

+

Output of analyses performed

+

+

+

  1. Side-by-side comparisons of current freely available image analysis programs. Comparisons made include the computer operating software compatibility requirements, platform used to carry out the functions of the program, image type that can be used, analysis capabilities, and the type of output of results.