Fig. 8

Oxamflatin and salermide together increase histone acetylation. a-b Representative Western blots of (a) histone H4 acetylated at lysines 5, 8, 12, and 16 (pan-H4ac) and (b) histone H4 acetylated specifically at lysine 16 (H4K16ac). Lysates are from genotyped 4 dpf larvae. c-d Quantification of Western blot analyses of levels of (c) pan-H4ac and (d) H4K16ac. Lysates were prepared from 4 replicate pools of animals for each treatment condition, with n = 4-10 animals per genotype per replicate. For each sample, pan-H4ac or H4K16ac signal was normalized to actin as a loading control. This value was then normalized to the WT + DMSO value within each set of samples. The plots show the average normalized signals of the 4 replicates. Error bars represent standard error. Significance was determined using a Student’s t test with Welch’s correction for unequal sample variances. In (c), *p < 0.05. In (d), *p = 0.015, **p = 0.003 compared to WT + DMSO control. Pan-H4ac levels are increased an average of 2.4× in oxamflatin+salermide-treated animals, and H4K16ac levels are increased an average of 1.6×