Fig. 2

CD82KO skeletal muscle shows delayed regeneration with smaller myofibers. a, b H&E representative images of regenerating WT and CD82^−/− muscles at 7 days. Comparisons of myofiber size at 7 days following CTX injury via minimum Feret’s diameter measurements (c) and myofiber distribution plots (d) show significant differences between the two strains using t tests. e, f H&E representative images of regenerating WT and CD82^−/− muscles at 21 days. Minimum Feret’s diameter comparisons (g) and myofiber distribution plots (h) at 21 days following CTX injury show significant differences between the two strains using t tests (i–k) Immunofluorescence images of entire sections stained for laminin (red) and embryonic MHC, which labels regenerating fibers (green). i is a control immunostaining of non-injured CD82^−/− muscle (no e-MHC+ fibers), whereas j and k are WT and CD82^−/− injured muscles 7 days following injury, respectively. l Graph of percentages of regenerating fibers in non-injured and cardiotoxin injured muscles. ***p < 0.001; ****p < 0.0001