Fig. 5

CD82^−/−:mdx^5cv satellite cells exhibit decreased activation potential compared to mdx^5cv. Satellite cell-derived cultures from mdx^5cv (a) and CD82^−/−:mdx^5cv (b) muscles stained for Pax7 (red) and MyoD (green) 72 h post isolation. Quantification plots of Pax7⁺MyoD⁻ (c) and Pax7⁺MyoD⁺ (d) cells at 24, 48, and 72 h post isolation in mdx^5cv and CD82^−/−:mdx^5cv revealed significant increase in Pax7⁺MyoD⁻ cells at 72 h post isolation in CD82^−/−:mdx^5cv cultures (***p < 0.001). Immunofluorescence staining of primary cultures from mdx^5cv (e) and CD82^−/−:mdx^5cv (f) mice using Pax7 (red) and Ki67 (green). Nuclei were counterstained with DAPI. g Quantification of Pax7⁺Ki67⁺ and h H2Aγ cells in mdx^5cv and CD82^−/−:mdx^5cv cultures revealed no significant differences at 24, 48, or 72 h post extraction. i–k Immunofluorescence staining and quantification of Pax7⁺ satellite cells in tissue sections from mdx^5cv and CD82^−/−:mdx^5cv mice. Pax7 staining is in red and laminin staining is in green. Satellite cells were counted in entire sections of the quadriceps but no significant difference between genotypes was observed. N = 6–10/genotype. Scale bars: 50 μm