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Fig. 6 | Skeletal Muscle

Fig. 6

From: Tetraspanin CD82 is necessary for muscle stem cell activation and supports dystrophic muscle function

Fig. 6

Loss of CD82 expression in dystrophic muscle leads to increased mTOR phosphorylation at Ser 2448. Quadriceps muscle extracts from 12-week-old male mdx5cv and CD82/:mdx5cv mice were analyzed by Western blot and probed for multiple signaling proteins known to control cell size and quantified. a Quantification of PTEN and pAKT (Ser 473) protein levels did not show significant differences between mdx5cv and CD82/:mdx5cv muscle extracts. b Immunoblots for mTOR (pSer 2448) and mTOR (total) show an increase of mTOR-phosphoSer2448 in CD82/:mdx5cv compared to mdx5cv muscles extracts (*p = 0.02). c Analyses of p-p70S6K and p4EBP1, two known targets of mTOR signaling normalized to their total non-phosphorylated forms show no significant differences between the genotypes. N = 5/genotype

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