Fig. 6From: Tetraspanin CD82 is necessary for muscle stem cell activation and supports dystrophic muscle functionLoss of CD82 expression in dystrophic muscle leads to increased mTOR phosphorylation at Ser 2448. Quadriceps muscle extracts from 12-week-old male mdx5cv and CD82−/−:mdx5cv mice were analyzed by Western blot and probed for multiple signaling proteins known to control cell size and quantified. a Quantification of PTEN and pAKT (Ser 473) protein levels did not show significant differences between mdx5cv and CD82−/−:mdx5cv muscle extracts. b Immunoblots for mTOR (pSer 2448) and mTOR (total) show an increase of mTOR-phosphoSer2448 in CD82−/−:mdx5cv compared to mdx5cv muscles extracts (*p = 0.02). c Analyses of p-p70S6K and p4EBP1, two known targets of mTOR signaling normalized to their total non-phosphorylated forms show no significant differences between the genotypes. N = 5/genotypeBack to article page