Fig. 10
From: Mitochondrial dysfunction and consequences in calpain-3-deficient muscle
Mitochondrial dependence of membrane repair efficiency after laser injury. Response to laser injury in Capn3-deficient (Capn3−/−) (blue) or WT (black) 3-month-old mice: biceps brachii (n = 10) (a), soleus (n = 10) (b), and EDL (n = 10) muscles (c) and EDL isolated fibers (n = 10) (d). Effect of using CCCP to uncouple the mitochondrial inner potential in response to laser injury in WT EDL muscle (red) and untreated EDL muscle (black) (n = 4 Capn3+/+, n = 5 Capn3+/+ + CCCP) (e). Response to laser injury in Capn3−/− EDL-isolated fibers after activation of mitochondrial activity with pyruvate (100 mM) (green) (n = 11), as compared to untreated Capn3−/− EDL isolated fibers (blue) (n = 3) (f). Inner membrane potential assessed with DiOC6 dye fluorescence in Capn3−/− EDL-isolated fibers after pyruvate treatment (n = 10), as compared to untreated Capn3−/− EDL muscle (n = 10) (g)