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Fig. 2 | Skeletal Muscle

Fig. 2

From: Mitochondrial dysfunction and consequences in calpain-3-deficient muscle

Fig. 2

Gene expression profiling of Capn3-deficient vs. Capn3-sufficient myoblasts with an Illumina BeadChip array. Dendrogram results attesting the good clustering of Capn3-deficient (Capn3−/−) myoblast samples when compared to corresponding WT (Capn3+/+) muscle cells using Partek Genomics Suite, a statistical analysis and interactive visualization software (a). Pathway analysis was done using the Ingenuity Pathways Analysis software that allows functional integration of molecular pathways. Ingenuity pathway analysis demonstrating changes in mitochondrial biogenesis, lipid metabolism, and protein transport in myoblasts. Pink indicates an upregulation and green indicates a downregulation of the specific gene in Capn3−/− myoblasts compared to Capn3+/+ myoblasts (b). Three different samples were used per group. cRNA was synthesized from 250 ng of total RNA for each sample. Gene pathways were prepared by ingenuity pathways analysis according to a gene list based on the interaction of a gene candidate with a p value of 0.001 and a fold increase ≥ 4

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