Fig. 4
From: Mitochondrial dysfunction and consequences in calpain-3-deficient muscle
Capn3 deficiency induces a mitochondrial metabolism deficiency in vitro. Live WT (Capn3+/+) and Capn3-deficient (Capn3−/−) myoblasts stained with DiOC6 to show their mitochondrial inner membrane potentials. Pictures were taken with the same focus, laser intensity, and exposure time in both panels (a). Flow cytometric analysis of 3,3′-dihexyloxacarbocyanine iodide (DiOC6) (mitochondrial inner membrane potential) in Capn3+/+ and Capn3−/− myoblasts (b). Histogram representing Capn3−/− (pink), Capn3+/+ (green), and Capn3+/+ and CCCP (uncoupler, purple) DiOC6 fluorescence (c). The DiOC6/NAO fluorescence ratio in Capn3+/+ and Capn3−/− myoblasts (d). The analysis was done on n = 6 flasks per group. ***Significantly different with p < 0.001