Fig. 1

CTX-induced injury in GM of WT and Nrf2^tKO animals. (a) Representative photos and (b) semi-quantitative assessment of GM damage based on H&E staining; n = 4-6. The activity of (c) CK and (d) LDH in plasma; activity assay; n = 4-5. (e) MCP-1 protein level in GM, Luminex^TM; n = 4-5. (f) Hmox1, (g) Il1b, (h) Il6 level in GM; qRT-PCR; n = 4-6. (i) Representative photos of microscopic assessment of myofiber necrosis in GM and (j) quantification of the staining; n = 4. The data are presented as mean +/− SEM; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001 vs. day 0; #p ≤ 0.05; ###p ≤ 0.001 vs. WT, one-way ANOVA with Tukey’s post hoc test. The scale bars represent 100 μm