Fig. 5

Infiltration of non-exercised WT, Nrf2^tKO, mdx, and Nrf2^tKOmdx hind limb muscle and diaphragm with leukocytes and macrophages. (a) Representative photos and (b) semi-quantitative analysis of inflammation in GM based on H&E staining; n = 4-6. (c) Representative photos and (d) semi-quantitative analysis of inflammation in diaphragm based on H&E staining; n = 4-6. (e) Hmox1 level in GM; qRT-PCR; n = 8-11. (f) Number of macrophages (CD45⁺F4/80⁺CD11b⁺); flow cytometry; n = 5; (g) representative two-parameters flow cytometry dot plots. (h) Number of M1-like macrophages (CD45⁺F4/80⁺CD11b⁺MHCII^hiCD206^lo) and (i) M2-like macrophages (CD45⁺F4/80⁺CD11b⁺MHCII^loCD206^hi); flow cytometry; n = 5; (j) representative two-parameters flow cytometry dot plots. The data are presented as mean +/− SEM; **p ≤ 0.01; ***p ≤ 0.001, one-way ANOVA with Tukey’s post hoc test. The scale bars represent 100 μm