Fig. 1
From: Dynamics of myogenic differentiation using a novel Myogenin knock-in reporter mouse
Generation of a Myog knock-in mouse line. a Scheme depicting the endogenous Myog locus, the donor construct and the result of the CRISPR-Cas9-mediated recombination in mouse embryonic stem cells. First-generation MyogntdTom-FNF mice were then crossed with a Tg(ACTFLPe)+/+ deleter strain to excise the FNF cassette. b Endogenous fluorescence from MyogntdTom/+ embryos at different stages. An overlay between the brightfield and fluorescent images is shown. Scale bar, 1000 μm. c Scheme showing the primer pairs amplifying the wild-type allele (2), the ntdTom allele (3) and both alleles (1) in the targeted Myog locus. RT-qPCR analysis of the levels of total Myog mRNA, the wild-type allele and the tdTom allele specifically from E14.5 Myog+/+, MyogntdTom/+ and MyogntdTom/ntdTom embryos. n = 4 embryos per genotype. Data represents mean ± s.d. Two-tailed unpaired Student’s t test; ***p value < 0.005, **p value = 0.0005 to 0.01, *p value = 0.01 to 0.05. d Western blot assessing the levels of MYOG and tdTOM proteins from E14.5 Myog+/+, MyogntdTom/+ and MyogntdTom/ntdTom embryos (n = 4 embryos per genotype). Bar graph shows the quantification of protein expression levels normalised to GAPDH. Data represents mean ± s.d. Two-tailed unpaired Student’s t test; ***p value < 0.005, *p value = 0.01 to 0.05