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Fig. 2 | Skeletal Muscle

Fig. 2

From: Dynamics of myogenic differentiation using a novel Myogenin knock-in reporter mouse

Fig. 2

tdTOM expression recapitulates endogenous Myog expression. a Whole-mount immunofluorescence from Myog+/+, MyogntdTom/+ and MyogntdTom/ntdTom embryos at E10.5 stained for tdTOM, MYOG and MyHC proteins. Scale bar, 300 μm on upper panels and 100 μm on lower insets. b Immunofluorescence of extraocular (EOM), tongue and limb muscle cryosections of MyogntdTom/+ embryos at E12.5. The bar graph shows the quantification of tdTOM- and MYOG-positive cells. n = 3 embryos, > 100 cells per muscle and per embryo were counted. Scale bar, 40 μm. c MuSCs were isolated from limb muscles of Tg:Pax7-nGFP; MyogntdTom adult animals and plated for in vitro differentiation for 5 days. Bar graph shows the quantification of tdTOM- and MYOG-positive cells (n = 3, > 200 cells per animal counted). Scale bar, 100 μm on the left image, 50 μm on the right inset

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