Fig. 3
From: Dynamics of myogenic differentiation using a novel Myogenin knock-in reporter mouse
Myog+ cells can be isolated based on tdTOM fluorescence. a FACS profiles of the mononuclear cell fraction isolated from limb muscles of foetal (E18.5), postnatal (p21) and adult MyogntdTom/+ animals. Bar graph shows the percentage of tdTOM+ cells at the different stages. n > 4 animals per condition. Data represents mean ± s.d. Two-tailed unpaired Student’s t test; **p value = 0.0005 to 0.01. b FACS profiles of the mononuclear cell fraction isolated from cardiotoxin injured TA muscles from Tg:Pax7-nGFP; MyogntdTom/+ mice at different timepoints (D3, D5, D10, D21 post-injury). c Immunostaining of the tdTOM+ cell fraction at 5 DPI isolated as in b for MYOG (3 h post-plating). Bar graph shows the quantification of tdTOM- and MYOG-positive cells (n = 3, > 200 cells per animal counted). Data represent mean ± s.d. Scale bar, 50 μm