Table 1 Antibodies used for immunostaining
From: Dynamics of myogenic differentiation using a novel Myogenin knock-in reporter mouse
| Species | Concentration | Reference | |
|---|---|---|---|
| Primary antibodies | |||
| Myog | Mouse monoclonal (IgG1) | 1:20 | DHSB, clone F5D, supernatant |
| Living colors® Ds-Red | Rabbit polyclonal | 1:500 | Takara Bio, 632496 |
| Myosin heavy chain (MyHC) | Mouse monoclonal (IgG2b) | 1:30 | DHSB, MF-20, supernatant |
| Secondary antibodies | |||
| Alexa Fluor®488 AffiniPure Goat Anti-Mouse IgG1(γ1) | Goat | 1:500 | Jackson ImmunoResearch Labs, 115-545-205 |
| Alexa Fluor® 555 F(ab')2 Fragment of Goat Anti-Rabbit IgG | Goat | 1:500 | Thermo Fisher Scientific, A-21430 |
| Alexa Fluor® 633 Goat Anti-Mouse IgG2b (γ2b) | Goat | 1:500 | Thermo Fisher Scientific, A-21146 |