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Table 2 Antibodies used for Western blot

From: Dynamics of myogenic differentiation using a novel Myogenin knock-in reporter mouse

 

Species

Concentration

Reference

Primary antibodies

 Myog

Mouse monoclonal (IgG1)

1:500

DHSB, clone F5D, supernatant

 Living colors® Ds-Red

Rabbit polyclonal

1:1000

Takara Bio, 632496

Secondary antibodies

 Mouse-HRP

Goat polyclonal

1:5000

Pierce, 31430

 Rabbit-HRP

Goat polyclonal

1:5000

Pierce, 31460

 GAPDH-Rhodamine

Human IgG Fab fragment

1:5000

Bio-Rad, 12004168