Fig. 3

Muscle atrophy in the ALAS2 transgenic mice. a Quadriceps cross section from hematoxylin and eosin stain. (bar, 50 μm). b Expression of eMyHC in WT and ALAS2 transgenic mice (Tg) muscle. (Bar of a-c is 50 μm, and bar of d-e 20 μm). c Single-fiber size comparison of WT and ALAS2 transgenic mice quadriceps muscle. d Quantification of average fiber diameter across single fibers in quadriceps muscle from WT (n = 4) and ALAS2 transgenic mice (n = 4). e Evans blue dye was intraperitoneal injected and mice sacked 24 h later. Transverse cross-sections of quadriceps muscle were observed with OLYMPUS BX51 equipped with green activation filters. Evans blue-positive fibers are denoted by red staining. Bar, 50 μm. f Serum LDH levels of WT (n = 7) and ALAS2 transgenic mice (n = 6). g Serum CK-MB levels of WT (n = 7) and ALAS2 transgenic mice (n = 6). h The relative mRNA expression of myod1, myogenin, and S6K1 (n = 5–8). i The relative mRNA expression of utrophin, dystrophin, Atrogin-1, and MuRF1. Values are means ± SD.*P < 0.05; **P < 0.01; ***P < 0.001