Fig. 1

Nanopattern surface promotes myotube and nuclei alignment. a Immunofluorescence images of 4-day-old myotubes on flat (left panel) or nanopatterned (ANFS, right panel) surface. Myotubes were stained using antibodies against α-actinin (green) and MEF2C (red) together with a DAPI staining (blue). Scale bar: 50 μm. b,c Quantification of fusion index (b) and MEF2C-positive nuclei (c). Data were obtained from four independent experiments, and each dot is the mean of 3 fields/dish. Error bars are Mean ± SEM. d Nuclei detection based on a pretrained model on flat (left panel) and on nanopattern (right panel) surface. Each cluster of nuclei is depicted with the same color code. e Corresponding distribution of the nuclei orientation on flat (left panel) and on nanopattern surface (right). f–g Median number of nuclei in cluster (f) and median eccentricity of the isolated nuclei (g). Perfect round nuclei would have a value of 0, and elongated nuclei have an eccentricity value closer to 1. Each dot is the median value of a field, from four to five independent experiments. Statistical analysis is based on Wilcoxon rank sum test: ^**** p = 5.76 × 10^-5 < α = 0.05, z = 4.023; ^**** p = 1.54 × 10^-13 < α = 0.05, z = − 7.384