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Fig. 3 | Skeletal Muscle

Fig. 3

From: Nanopattern surface improves cultured human myotube maturation

Fig. 3

Expression of EC coupling-associated proteins and myosin heavy chain during myotube maturation. a Transcript levels of genes encoding for proteins involved in muscle contraction and EC coupling were quantified by RT-qPCR in adult muscle (three donors) and in myoblasts (D0), 4-day-old (D4), and 10-day-old (D10) myotubes (cultures from five donors). CACNA1S (Cav1.1), RYR1 (RyR1), ATP2A1 (SERCA1), ATP2A2 (SERCA2), MYH1 (MyHC-2X), MYH2 (MyHC-2A), MYH3 (MyHC-3 embryonic) and MYH7 (MyHC-7 slow). Transcript levels are relative to adult muscle transcript content. Results are mean ± SEM. A.U., arbitrary units. Each dot represents one independent experiment. b PCR products of the two variants of Cav1.1, embryonic (Cav1.1e) and adult (Cav1.1a) detected in 4- and 10-day-old myotubes and in human adult muscle. M, marker of molecular weights. c PCR product sequencing of CACNA1S exon 29, specific of the adult isoform of Cav1.1 (Cav1.1a) but absent in the embryonic isoform (Cav1.1e). d Quantification of the ratio between Cav1.1e and Cav1.1a expressions in 4- and 10-day-old myotubes. Error bars represent mean ± SEM

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