Fig. 6

Ca²⁺ transients upon electric field stimulation. a Representative traces of cytosolic Ca²⁺ transients generated by repeated burst of 1 s at 10 Hz, every 10 s for 3 min in 4-day (left panel) and 10-day (right panel)-old myotubes, differentiated on nanopattern surface. Statistical evaluation of the amplitude (b) and the decay time (c) of the first Ca²⁺ transient, and the percentage of remaining calcium at the end of 3-min stimulation (d). Data were obtained from four independent experiments, and each dot is a mean of 4−6 myotubes on a field. Error bars are mean ± SEM