Table 1 Cell culture media
From: Nanopattern surface improves cultured human myotube maturation
Concentration | Reference | |
|---|---|---|
Growth medium (GM) | ||
F-10 (Ham’s) | ThermoFisher 31550-023 | |
BSA | 0.5 mg/ml | Sigma 05482 |
Insulin | 0.04 mg/ml | I-9278 |
Creatine powder | 1 mM | Fluka 27900 |
Dexamethasone | 0.39 μg/ml | Sigma D1756 |
Gentamycin | 5 μg/ml | Sigma G1272 |
EGF | 10 ng/ml | Corning 354001 |
FBS | 15% | Lot 4205662K |
Fetuin | 0.5 mg/ml | Sigma F-3385 |
Sodium pyruvate | 100 μg/ml | Sigma P4562 |
Uridine | 50 μg/ml | Sigma U3003 |
Differentiation medium (DM) | ||
DMEM | Thermo Fisher 41965-039 | |
BSA | 0.5 mg/ml | Sigma 05482 |
Insulin | 0.01 mg/ml | Sigma I5500 |
Creatine powder | 1 mM | Fluka 27900 |
Gentamycin | 10 μg/ml | Thermo Fisher 15710-049 |
Sodium pyruvate | 100 μg/ml | Sigma P4562 |
Uridine | 50 μg/ml | Sigma U3003 |
Horse serum | 1% | Amimed 2-05F26-I |