Table 2 List of primary and secondary antibodies
From: Nanopattern surface improves cultured human myotube maturation
Dilution | Reference | |
|---|---|---|
Primary antibodies | ||
Mouse anti-(sarcomeric) α-actinin | 1:500 | Sigma A7811 |
Mouse anti-Pax7 | 1:100 | DSHB C |
Mouse anti-RyR1 | 1:500 | DSHB 34C |
Rabbit anti-MEF2C | 1:500 | Cell Signaling 5030S |
Rabbit anti-STIM1 (C-terminal) | 1:500 | Sigma S6197 |
Rabbit anti-MyoD1 | 1:200 | Cell Signaling D8G3 |
Secondary antibodies: | ||
Alexa Fluor® 488-conjugated goat anti-mouse IgG (H + L) | 1:1000 | Life Technologies A11029 |
Alexa Fluor® 546-conjugated goat anti-mouse IgG (H + L) | 1:1000 | Life Technologies A11030 |
Alexa Fluor® 546-conjugated goat anti-rabbit IgG (H + L) | 1:1000 | Life Technologies A11030 |