Table 3 List of primers
From: Nanopattern surface improves cultured human myotube maturation
Proteins | Names | Sequences | |
|---|---|---|---|
Genes | |||
CACNA1S | Cav1.1α1s | 26-35F | CAC CTC CTC CTA CTT TGA ATA |
26-35R | AGA ACT TCC CAA AGC CCA GA | ||
E27F | GCT CAT GGC CTT CAA GG | ||
ex31R | TGA CGA TGA GCA GAG CC | ||
RYR1 | RyR1 | ex-102-103-27F | TGG CCA TCA TCC AGG GTC T |
ex-102-103-77R | GGT CTC GGA GCT CAC CAA AAG | ||
ATP2A1 | SERCA1 | ex-15-16-29F | CAG TGG CTG GCT CTT CTT CC |
ex-15-16-79R | GCA CCC ACA TAG CCC CC | ||
ATP2A2 | SERCA2 | ex-3V-871F | CCT TGA GGA CTC TGC CAA CTT T |
ex-3V-921R | ACG AAG GTC AGA TTG GTC TCA TATT | ||
MYH1 | MyHC-2X (fast) | ex-39-40-48F | CAA GCT GAA GAA GCG GAG GA |
ex-39-40-98R | GCG GAA TTT GGA GAG GTT GAC | ||
MYH2 | MyHC-2A (fast) | ex-37-38-64F | AAA CTG GAG GCC AGG GTA CG |
ex-37-38-114R | TTG CTC ACT CTC AAC CTC TCC TT | ||
MYH3 | MyHC-3 (embryonic) | 7F | TCA GAA GCC GAT TCT ACA TGG AC |
57R | ACA ACT TAG CGG CAC TTG GG | ||
MYH7 | MyHC-7 (slow) | ex-37-38-66F | AGG AGC TCA CCT ACC AGA CGG |
ex-37-38-116R | GCA GCC GCA GCA GGT TT | ||
Housekeeping genes | |||
β2-microglobulin | TGC TCG CGC TAC TCT CTC TTT | ||
TCT GCT GGA TGA CGT GAG TAA AC | |||
EEF1A1 | AGC AAA AAT GAC CCA CCA ATG | ||
GGC CTG GAT GGT TCA GGT A | |||