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Fig. 3 | Skeletal Muscle

Fig. 3

From: Single cell RNA-seq analysis of the flexor digitorum brevis mouse myofibers

Fig. 3

RISH staining of variably expressed genes across extensor digitorum longus (EDL), gastrocnemius (GC), and soleus (SOL). a–c Fhl1 (green) and Eno3 (red) show differing expression patterns across EDL (a), GC (b), and SOL (c). There is reduced Fhl1 in the fast-twitch GC and increased Fhl1 in the slow-twitch SOL fiber. d–f Fhl1 (green) is coexpressed with Myh2 (red), which has a perinuclear pattern. Both Fhl1 and Myh2 are reduced in GC (e) and increased in SOL (f). g–i Got2 (green) is coexpressed with Myh2 (red) showing highest expression in the EDL (g). j Neuronal tissue showing strong staining of Gabbr2 and Ntrk3. k Gabbr2 shows a variable blush across the GC, while no discernable Ntrk3 was observed in the GC. Nuclei were stained with DAPI (blue) in all images

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