Fig. 4From: mRNP granule proteins Fmrp and Dcp1a differentially regulate mRNP complexes to contribute to control of muscle stem cell quiescence and activationDifferential expression of mRNP granule proteins in proliferating, quiescent and differentiated muscle cells in culture. a Schematic depicts segregation of transcripts into translating and non-translating pools on emergence from the nucleus with a constellation of RNA-binding proteins. Non-translated transcripts may be sequestered in mRNPs enriched for decay complex (mRNA turnover) or storage granule components (translational repression/stabilization of mRNA). b Western blot analysis showing that three distinct cellular states can be distinguished by expression of Myogenin and Cyclin D1 (MB: asynchronously proliferating myoblasts are CycD1+, MyoG-; MT: 5-day differentiated myotubes are CycD1-, MyoG+; G0: quiescent myoblasts are CycD1-, MyoG-). c Western blot profile of mRNP granule protein expression across three cellular states. MB: proliferating myoblasts; G0: quiescent myoblasts; MT: differentiated myotubes. Expression of most proteins is suppressed in G0; notable exceptions are Fmrp and Tia1, which are maintained in G0 (see Table 1 and quantification in Fig. 4d). d Quantification of relative expression of mRNP granule proteins across the three cellular states calculated from densitometric analysis of immuno-blots. Each protein was normalized to Gapdh in the same sample, before normalizing to MB. Values represent mean + SD, n = 3, two-tailed paired Student’s t tests are indicated as [* p ≤ 0.05, ** p ≤ 0.01]Back to article page