Skip to main content

Table 3 Details of antibodies used in this study

From: mRNP granule proteins Fmrp and Dcp1a differentially regulate mRNP complexes to contribute to control of muscle stem cell quiescence and activation

Antibody Species Company Catalog # Dilution for WB Dilution for IFA
Argonaute2 Rabbit CST C34C6 1 in 1000 1 in 200
Cyclin A2 Rabbit Abcam ab181591 1 in 2000  
Cyclin E Rabbit Abcam ab71535 1 in 1000  
Dcp1a Mouse Santa Cruz sc100706 1 in 200 1 in 50
Edc3 Mouse Santa Cruz sc365024 1 in 200 1 in 50
Edc4 Rabbit Santa Cruz ab72408 1 in 200 1 in 50
eIF4E Mouse Santa Cruz sc271480 1 in 200 1 in 50
Eif4g Goat Santa Cruz sc9602 1 in 200 1 in 50
Fmrp Rabbit Sigma 4055 1 in 1000 1 in 200
FxR1 Goat Abcam ab51970 1 in 1000  
Gapdh Mouse Abcam ab8245 1 in 10000  
GFP Chicken Abcam ab13970   1 in 300
GW182 Mouse Santa Cruz sc56314 1 in 200 1 in 50
γH2AX (Ser139) Mouse Upstate 05636   1 in 200
MyoD Mouse Dako M3512 1 in 250 1 in 200
MyoG Mouse Santa Cruz sc12732 1 in 250 1 in 250
Myosin Heavy Chain Mouse Hybridoma A4.1025 1 in 1 1 in 1
Pabp1 Rabbit Abcam ab21060   1 in 50
Pax7 Mouse Aviva ARP30947_P050 1in 100  
RLP0 Rabbit Abcam ab101279 1 in 1000  
Tia1 Mouse Santa Cruz sc166247 1 in 200 1 in 50
TiaR Goat Santa Cruz sc1749 1 in 200 1 in 50
Xrn1 Rabbit Sigma sab4200028 1 in 1000 1 in 200