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Fig. 2 | Skeletal Muscle

Fig. 2

From: Transcription factor signal transducer and activator of transcription 6 (STAT6) is an inhibitory factor for adult myogenesis

Fig. 2

STAT6 overexpression impaired myoblast fusion. a Representative images of Western blot bands for p-STAT6 and GAPDH. Myoblasts were infected with Ad-Ctrl or Ad-STAT6 adenovirus vector in growth medium (GM). After 24 h, the medium was replaced with a differentiation medium and maintained for 48 h. The cells were then used for Western blot and immunocytochemical analysis. b Relative expression of total STAT6. n = 6. *p < .05 vs. Ad-Ctrl. c Relative expression of phosphorylated STAT6. n = 6. *p < .05 vs. Ad-Ctrl. d Representative immunostained myotubes positive for MyHC (green). Nuclei were stained with DAPI (blue). Scale bar = 50 μm. e Calculation of the fusion index in the Ad-Ctrl and Ad-STAT6 treatments. f Diameters of myotubes in the Ad-Ctrl and Ad-STAT6 treatments. g Percentages of myosin-positive cells with one, two, and three or more nuclei. n = 6 in each group. *p < .05 vs. Ad-Ctrl. Data are presented as mean ± SD

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