Fig. 3.

STAT6 knockdown improved myoblast fusion. Myoblasts were transfected with empty (Ctrl) or shSTAT6 vectors and grown in GM. After 48 h, the cells were harvested to ascertain p-STAT6, STAT6, and GAPDH levels via Western blotting. a representative images of Western blot bands. Relative protein expression levels of b STAT6 and c p-STAT6. n = 6 per group. *p < .05 vs. Ctrl. d representative immunostained myotubes positive for MyHC (green). Nuclei were stained with DAPI (blue). Scale bar = 50 μm. e Calculation of fusion index in the Ctrl and shSTAT6 treatment. n = 4. f Diameters of myotubes in the Ctrl and shSTAT6 treatments. g Percentages of myosin-positive cells. n = 4. *p < .05 vs. Ctrl. Data are presented as mean ± SD