Fig. 3

Amiloride profoundly ameliorated gastrocnemius atrophy in the CT26 model and the LLC model. Cancer cachexia features of gastrocnemius were analyzed for the NOR, CAC, AM, and KD mice on day 30. a, b Representative microscope pictures of hematoxylin and eosin staining for gastrocnemius of the CT26 model (a) and the LLC model (b) (scale bar = 50 μm). c, d Myofiber size distributions of gastrocnemius of the CT26 model (c) and the LLC model (d) (n = 5, 200 myofibers were used). e Expressions of MuRF-1, Atrogin-1, MyoD1, and Myogenin proteins in gastrocnemius of the CT26 and LLC models. f Quantification of the expressed proteins (n = 4). Statistical significances: p > 0.05, NS; p < 0.05, *; p < 0.01, **; p < 0.001, ***; p < 0.0001, ****. NOR, C57BL/6 or BALB/c normal control mice; CAC, CT26/LLC cachexia mice; AM, amiloride-treated mice; KD, mice inoculated with Rab27-knockdown CT26/LLC cells