Fig. 4

Simvastatin treatment does not influence muscle regeneration in gastrocnemius muscle of mdx mice. A, B Quantification of muscle fiber size based on laminin staining (not shown); n=5–6/group; mean ± SEM. C The quantitative analysis and representative photos of centrally nucleated fibers (CNF) performed based on H&E staining showing a drop in CNF number in mdx animals treated with simvastatin when compared to the vehicle group; scale bar: 100 μm; n=5–6/group; mean ± SEM. D Expression of myosin heavy chain isoform-coding gene: Myh3, presented as mean ± SEM; n=10–13; qRT-PCR. E The protein level of FGF2 in the gastrocnemius muscle of dystrophic animals upon simvastatin treatment; n=10–13/group; presented as mean ± SEM; ELISA. F myomiRs: miR-1, miR-133a-3p, and miR-206 expression upon simvastatin treatment in gastrocnemius muscle of mdx animals presented as mean ± SEM; n=12–14; WT level marked with the dotted line; LNA qRT-PCR. G The quantitative analysis and representative photos of Pax7-positive satellite cells in the gastrocnemius muscle; scale bar: 100 μm; n=9–12/group; mean ± SEM; ^*for mdx simvastatin vs. mdx vehicle and ^$for mdx vehicle vs. WT comparison; ^*p < 0.05, ^***p < 0.001, ^****p < 0.0001, ^$$$p < 0.001, ^$$$$p < 0.0001