Fig 1

Plating on Matrigel increases proliferation of primary human myoblasts (skMDCs). A Representative phase contrast images of untreated cells in Matrigel coated (Mat^pos; iv, v, vi) and uncoated (Mat^neg ; i, ii, iii) wells at 24, 48, and 72 h after seeding (~ 6.8 × 10³ cells/cm²). B Proliferation rate of three skMDC lines, relative to the number of cells plated (value = 1 at 0 h). Data are reported as mean ± SD. Statistics were performed using a two-way repeated measures ANOVA with Sidak’s multiple comparison. n = 5 (1× 18 M, 1× 32F, 3× 42F); ** = p < 0.01. Scale bar = 1 mm